Antibacterial Effect of Zinc Oxide Nanoparticles on Standard Strains and Isolates of Pseudomonas Aeruginosa and Staphylococcus Aureus

Background: Studies have shown that metal nanoparticles are highly active and exhibits remarkable bactericidal activity against a wide range of bacteria. Objective: The aim of this study was to examine the antibacterial activity of zinc oxide nanoparticles against standard strains of Pseudomonas aeruginosa and Staphylococcus aureus and their isolates in food products. Methods: This experimental study was conducted on the two pathogenic bacteria and their two standard strains. Zinc oxide nanoparticles were prepared from zeolite and their amount was determined using the XRF analyzer. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured using disk diffusion method . Findings: The MIC value of zinc oxide nanoparticles was 4 mg/mL for standard strain and isolate of pseudomonas aeruginosa and 2 mg/mL for standard strain and isolate of staphylococcus aureus. The MBC values for standard strain and isolate of pseudomonas aeruginosa were 16 and 8 mg/mL, respectively, while for the standard strain and isolate of Staphylococcus aureus it was reported 8 mg/mL. Conclusion: Staphylococcus aureus is more sensitive to zinc oxide nanoparticles that pseudomonas aeruginosa.